The Tissue Engineered Mechanics, Imaging and Materials laboratory (TEMIM LAB) primary research focus lies in the area of cell and engineered tissue mechanics with application in cardiovascular regenerative medicine. The TEMIM lab, conducts both experimental and computational investigations in this area. A major goal of the lab is to develop functional tissue engineered heart valves (TEHVs) using 1) porcine small intestinal submucosa (PSIS) substrates and 2) mechanically regulate stem cells for the TEHV application as well as for (3) broader application in cardiovascular regenerative medicine. Concurrently the TEMIM lab is also working towards the elucidation of mechanobiological cellular and molecular mechanisms that are involved in the etiology of valve diseases, particularly aortic valve calcification. A specific project in this area involves (4) the delineation of mechanosensitive fluid and structural conditions of the aortic valve due to elastin remodeling that may serve as an early indicator of calcific aortic valve disease (CAVD). In addition, at the cellular level, the lab is interested in identifying the fluid-induced mechanobiological responses of valve endothelial cells in valve homeostasis and in the development of CAVD. The research in the TEMIM lab has been supported by the AHA, the Miami Heart Research Institute, NSF, industry and academic funding sources.